CABIN1 antibody (pAb)

RRID: AB_2793694
Aliases: CAIN, PPP3IN
Catalog No: 61597 Format: 100 µl ¥86,000 Buy
Catalog No: 61598 Format: 10 µl ¥24,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
280 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
IP: 10 µl per IP
WB: 1:500 dilution

Individual optimization may be required.

Immunogen

This antibody was raised against a peptide within the C-terminal region of human CABIN1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
CABIN1 antibody (pAb) tested by Western blot.

CABIN1 antibody (pAb) tested by Immunoprecipitation / Western blot.
10 µg of HIRA antibody (mAb), Cat. No. 39555, (lane 1) or 10 µl of CABIN1 antibody (pAb) (lane 2) was used to immunoprecipitate CABIN1 from 1mg of U2OS nuclear extract. 10 µg of rabbit IgG was also used as a negative control (lane 3). The immunoprecipitated protein was detected by Western blotting using CABIN1 antibody at a dilution of 1:500.

Background

CABIN1 (calcineurin-binding protein 1) may be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. Component of a complex that includes at least ASF1A, CABIN1, HIRA, Histone H3.3 and UBN1.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting