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NRF2 antibody (pAb)

Aliases: NFE2L2
商品コード: 61599 Format: 100 µl ¥69,000 Buy Now
商品コード: 61600 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:96 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for NRF2 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:500 - 1:1,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

for NRF2 antibody (pAb)Immunogen

This antibody was raised against a peptide within human NRF2.

for NRF2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NRF2 antibody (pAb)References

 
NRF2 antibody (pAb) tested by ChIP-Seq.

NRF2 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from A549 cells and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify NRF2 binding sites. The image shows binding across regions of chromosome 16 and 22. You can view the complete data set in the UCSC Genome Browser, starting at NQO1, here.

NRF2 antibody (pAb) tested by Western blot.

NRF2 antibody (pAb) tested by Western blot.
Nuclear extract of HepG2 cells (25 µg) treated with tHBQ and probed with NRF2 antibody at a dilution of 1:500.

for NRF2 antibody (pAb)Background

NRF2 (NF-E2-Related Factor 2) is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with NRF2 antibody (pAb).

for NRF2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NRF2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NRF2 antibody (pAb)
 

Data Thumbnails

NRF2 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of NRF2 pAb.
(Click image to enlarge and see details.)

NRF2 antibody (pAb) tested by Western blot.

Western blot of NRF2 pAb.
(Click image to enlarge and see details.)