IFNA1 antibody (mAb)

RRID: AB_2793697
Aliases: IFL, IFN, IFNA@, IFNA13, IFN-ALPHA, IFN-alphaD
Catalog No: 61603 Format: 100 µg ¥86,000 Buy
Catalog No: 61604 Format: 10 µg ¥24,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
19 kDa
Reactivity:
Human

Applications

Enzyme-linked Immunosorbent Assay Validated

Application Notes

Applications Validated by Active Motif:
ELISA: Individual optimization may be required.

Immunogen

Human IFNA1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

IFNA1 antibody (mAb) tested by ELISA.
Recombinant IFNA1 protein or a negative control protein was conjugated to Luminex® microspheres and then incubated in the same well with a serial dilution of IFNA1 antibody (mAb).  Biotinylated anti-mouse IgG followed by Streptavidin-PE was used to detect the bound IFNA1 antibody . Mean Fluorescence Intensity (MFI) for each protein was measured in a Luminex® 200™.

Background

IFNA1 (Interferon, Alpha 1) is produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting