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STAT3 antibody (pAb)

Aliases: APRF, HIES, ADMIO
商品コード: 61645 Format: 100 µl ¥67,000 Buy Now
商品コード: 61646 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:96 kDa
Reactivity:Human

Applications

Western Blot Validated

for STAT3 antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:2,500 dilution

for STAT3 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human STAT3.

for STAT3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT3 antibody (pAb)References

 
STAT3 antibody (pAb) tested by Western blot.

STAT3 antibody (pAb) tested by Western blot.
Detection of STAT3 by Western blot analysis. Nuclear extract of HepG2 cells (30 µg) probed with STAT3 antibody at a dilution of 1:2,000.

for STAT3 antibody (pAb)Background

STATs (signal transducers and activators of transcription) are a family of 7 transcription factors that form part of the JAK-STAT signaling cascade. This cascade is the basis of the signal transduction mechanism for many cytokine receptors. Once activated by phosphorylation by JAKs, STATs translocate to the nucleus. Accumulation of STATs in the nucleus is both rapid and tightly controlled. A number of factors regulate the JAK-STAT pathway including STAT dephosphorylation by phosphatases, altered nuclear import-export dynamics of STAT, and STAT gene activation antagonists such as SOCS (suppressors of cytokine signaling) and PIAS (Protein Inhibitors of Activated STATs).

for STAT3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT3 antibody (pAb)
 

Data Thumbnails

STAT3 antibody (pAb) tested by Western blot.

Western blot of STAT3 pAb.
(Click image to enlarge and see details.)