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STAT2 antibody (pAb)

Aliases: P113, ISGF-3, STAT113
商品コード: 61651 Format: 100 µl ¥67,000 Buy Now
商品コード: 61652 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:110 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for STAT2 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

for STAT2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human STAT2.

for STAT2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT2 antibody (pAb)References

 
STAT2 antibody (pAb) tested by immunoprecipitation.

STAT2 antibody (pAb) tested by Immunoprecipitation.
10 µl of STAT2 antibody was used to immunoprecipitate STAT2 from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the STAT2 antibody at a dilution of 1:1,000.

STAT2 antibody (pAb) tested by Western blot.

STAT2 antibody (pAb) tested by Western blot.
Whole cell extract (20 µg) of Jurkat cells (Lane 1) or HeLa cells (Lane 2) probed with STAT2 antibody at a 1:1,000 dilution.

for STAT2 antibody (pAb)Background

STAT2 (Signal Transducer And Activator Of Transcription 2, 113kDa) is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

for STAT2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT2 antibody (pAb)
 

Data Thumbnails

STAT2 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of STAT2 pAb.
(Click image to enlarge and see details.)

STAT2 antibody (pAb) tested by Western blot.

Western blot of STAT2 pAb.
(Click image to enlarge and see details.)