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NCOA1 antibody (pAb)

Aliases: SRC1, KAT13A, RIP160, F-SRC-1, bHLHe42, bHLHe74
商品コード: 61653 Format: 100 µl ¥67,000 Buy Now
商品コード: 61654 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:170 kDa
Reactivity:Human

Applications

Western Blot Validated

for NCOA1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NCOA1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human NCOA1.

for NCOA1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NCOA1 antibody (pAb)References

 
NCOA1 antibody (pAb) tested by Western blot.

NCOA1 antibody (pAb) tested by Western blot.
Nuclear extract of HEK293 cells (20 µg) probed with NCOA1 antibody at a dilution of 1:500.

for NCOA1 antibody (pAb)Background

NCOA1 (Nuclear Receptor Coactivator 1) is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

for NCOA1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NCOA1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NCOA1 antibody (pAb)
 

Data Thumbnails

NCOA1 antibody (pAb) tested by Western blot.

Western blot of NCOA1 pAb.
(Click image to enlarge and see details.)