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ATF4 antibody (pAb)

Aliases: CREB2, TXREB, CREB-2, TAXREB67
商品コード: 61655 Format: 100 µl ¥67,000 Buy Now
商品コード: 61656 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:42 kDa
Reactivity:Human

Applications

Western Blot Validated

for ATF4 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for ATF4 antibody (pAb)Immunogen

This antibody was raised against a recombinant protein to human ATF4.

for ATF4 antibody (pAb)Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

for ATF4 antibody (pAb)References

 
ATF4 antibody (pAb) tested by Western blot.

ATF4 antibody (pAb) tested by Western blot.
ATF4 detection by Western blot. The analysis was performed using U2Os whole cell extract (30 µg) and ATF4 antibody at a 1:500 dilution.

for ATF4 antibody (pAb)Background

ATF4 (Activating Transcription Factor 4) is a transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4.

for ATF4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATF4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ATF4 antibody (pAb)
 

Data Thumbnails

ATF4 antibody (pAb) tested by Western blot.

Western blot of ATF4 pAb.
(Click image to enlarge and see details.)