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RNA pol II antibody (mAb)

Clone: 1F4B6
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
商品コード: 61667 Format: 100 µg ¥69,000 Buy Now
商品コード: 61668 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:240 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for RNA pol II antibody (mAb) (Clone 1F4B6)Application Notes


Validated Applications:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
ICC/IF: 5 µg/ml dilution
WB: 0.5 - 2 µl/ml dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for RNA pol II antibody (mAb) (Clone 1F4B6)Immunogen

This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.

for RNA pol II antibody (mAb) (Clone 1F4B6)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RNA pol II antibody (mAb) (Clone 1F4B6)References

 
RNA pol II antibody (mAb) tested by ChIP-Seq.

RNA pol II antibody (mAb) (Clone 1F4B6) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from HL-60 cells and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify RNA pol II binding sites. The image shows binding across a region of chromosome 15. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

RNA pol II antibody (mAb) tested by immunofluorescence.

RNA pol II antibody (mAb) (Clone 1F4B6) tested by immunofluorescence.
Left: HeLa cell stained with RNA pol II antibody (mAb). Middle:Hoechst. Right: Merge.

RNA pol II antibody (mAb) tested by Western blot.

RNA pol II antibody (mAb) (Clone 1F4B6) tested by Western blot.
HeLa nuclear extract (30 µg per lane) probed with RNA pol II antibody (mAb) at a 1 µg/ml dilution.

for RNA pol II antibody (mAb) (Clone 1F4B6)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA pol II antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with RNA pol II antibody (mAb).

for RNA pol II antibody (mAb) (Clone 1F4B6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA pol II antibody (mAb) (Clone 1F4B6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA pol II antibody (mAb)
 

Data Thumbnails

RNA pol II antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of RNA pol II mAb.
(Click image to enlarge and see details.)

RNA pol II antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of RNA pol II mAb.
(Click image to enlarge and see details.)

RNA pol II antibody (mAb) tested by Western blot.

Western blot of RNA pol II mAb.
(Click image to enlarge and see details.)