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DMAP1 antibody (pAb)

Aliases: EAF2, SWC4, MEAF2, DNMAP1, DNMTAP1
商品コード: 61675 Format: 100 µl ¥67,000 Buy Now
商品コード: 61676 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for DMAP1 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

for DMAP1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human DMAP1.

for DMAP1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DMAP1 antibody (pAb)References

 
DMAP1 antibody (pAb) tested by immunoprecipitation.

DMAP1 antibody (pAb) tested by Immunoprecipitation.
10 µl of DMAP1 antibody was used to immunoprecipitate DMAP1 from 500 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DMAP1 antibody at a dilution of 1:500.

DMAP1 antibody (pAb) tested by Western blot.

DMAP1 antibody (pAb) tested by Western blot.
Nuclear extract (30 µg) of Caco-2 (Lane 1) and HeLa cells (Lane 2) probed with DMAP1 antibody at a 1:500 dilution.

for DMAP1 antibody (pAb)Background

DMAP1 (DNA Methyltransferase 1 Associated Protein 1) is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

for DMAP1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DMAP1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DMAP1 antibody (pAb)
 

Data Thumbnails

DMAP1 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of DMAP1 pAb.
(Click image to enlarge and see details.)

DMAP1 antibody (pAb) tested by Western blot.

Western blot of DMAP1 pAb.
(Click image to enlarge and see details.)