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CXXC4 antibody (pAb)

Aliases: Idax, 9330210J02Rik, C030003J12Rik
商品コード: 61679 Format: 100 µl ¥67,000 Buy Now
商品コード: 61680 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:42 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for Cxxc4 antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:2,500 dilution

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Cxxc4 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of mouse Cxxc4.

for Cxxc4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Cxxc4 antibody (pAb)References

 
Cxxc4 antibody (pAb) tested by Western blot.

Cxxc4 antibody (pAb) tested by Western blot.
Detection of Cxxc4 antibody by Western blot analysis.
Lane 1: Mouse ES cytoplasmic extract.
Lane 2: F9 cytoplasmic extract.
Lane 3: P19 cytoplasmic extract.
Lane 4: HEK293 whole cell extract.
30 µg of lysate per lane probed with Cxxc4 antibody at a dilution of 1:1,000.

for Cxxc4 antibody (pAb)Background

Cxxc4 (CXXC Finger Protein 4) acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1 and has been implicated in malignant renal cell carcinoma and colonic villous adenoma. Recently, Cxxc4 has been shown to be involved in the regulation of DNA demethylation. It binds to unmethylated CpGs in DNA and is involved in TET2 downregulation.

for Cxxc4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Cxxc4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CXXC4 antibody (pAb)
 

Data Thumbnails

Cxxc4 antibody (pAb) tested by Western blot.

Western blot of Cxxc4 pAb.
(Click image to enlarge and see details.)