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NCAPH2 antibody (mAb)

Clone: 5F2G4
Aliases: CAPH2
商品コード: 61681 Format: 100 µg ¥67,000 Buy Now
商品コード: 61682 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:90 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for NCAPH2 antibody (mAb) (Clone 5F2G4)Application Notes


Validated Applications:
ICC/IF: 1 - 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NCAPH2 antibody (mAb) (Clone 5F2G4)Immunogen

This antibody was raised against a recombinant protein to human NCAPH2.

for NCAPH2 antibody (mAb) (Clone 5F2G4)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NCAPH2 antibody (mAb) (Clone 5F2G4)References

 
NCAPH2 antibody (mAb) tested by immunofluorescence.

NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence.
Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.

NCAPH2 antibody (mAb) tested by Western blot.

NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot.
NCAPH2 antibody detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear extract and NCAPH2 antibody at a 1 µg/ml dilution.

for NCAPH2 antibody (mAb) (Clone 5F2G4)Background

NCAPH2 (Non-SMC Condensin II Complex, Subunit H2) Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. (By similarity)

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with NCAPH2 antibody (mAb).

for NCAPH2 antibody (mAb) (Clone 5F2G4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NCAPH2 antibody (mAb) (Clone 5F2G4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NCAPH2 antibody (mAb)
 

Data Thumbnails

NCAPH2 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of NCAPH2 mAb.
(Click image to enlarge and see details.)

NCAPH2 antibody (mAb) tested by Western blot.

Western blot of NCAPH2 mAb.
(Click image to enlarge and see details.)