Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

CHD5 antibody (mAb)

Clone: 5A10
Aliases: CHD-5, AW060752, 4930532L22Rik, B230399N07Rik
商品コード: 61683 Format: 100 µg ¥67,000 Buy Now
商品コード: 61684 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:260 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Chd5 antibody (mAb) (Clone 5A10)Application Notes


Validated Applications:
ICC/IF: 2 - 5 µg/ml dilution
WB: 1 - 2 µg/ml dilution

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Chd5 antibody (mAb) (Clone 5A10)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse Chd5.

for Chd5 antibody (mAb) (Clone 5A10)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Chd5 antibody (mAb) (Clone 5A10)References

 
Chd5 antibody (mAb) tested by immunofluorescence.

Chd5 antibody (mAb) (Clone 5A10) tested by immunofluorescence.
Left: HeLa cells. Right: E12.5 mouse gut. Both stained with Chd5 antibody.

Chd5 antibody (mAb) tested by Western blot.

Chd5 antibody (mAb) (Clone 5A10) tested by Western blot.
Chd5 antibody detection by Western blot. The analysis was performed using 40 µg of HeLa nuclear extract and Chd5 antibody at a 2 µg/ml dilution.

for Chd5 antibody (mAb) (Clone 5A10)Background

Chd5 (Chromodomain Helicase DNA Binding Protein 5) is member of the CHD subfamily of chromatin remodeling SWI/SNF proteins, containing chromodomains and a SNF2-like ATPase/helicase domain, that play a role in chromatin remodeling and gene transcription. Chd5 is also a potential tumor suppressor that may play a role in neuronal development.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CHD5 antibody (mAb).

for Chd5 antibody (mAb) (Clone 5A10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Chd5 antibody (mAb) (Clone 5A10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CHD5 antibody (mAb)
 

Data Thumbnails

Chd5 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Chd5 mAb.
(Click image to enlarge and see details.)

Chd5 antibody (mAb) tested by Western blot.

Western blot of Chd5 mAb.
(Click image to enlarge and see details.)