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RNA pol II CTD phospho Ser2 antibody (mAb)

Clone: 3E7C7
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
商品コード: 61699 Format: 100 µg ¥67,000 Buy Now
商品コード: 61700 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:240 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Application Notes


Validated Applications:
ICC/IF: 4 - 6 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Immunogen

This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)References

 
RNA pol II CTD phospho Ser2 antibody (mAb) tested by immunofluorescence.

RNA pol II phospho Ser2 antibody (mAb) (Clone 3E7C7) tested by Immunofluorescence.
Left: HeLa cell stained with RNA pol II CTD phospho Ser2 antibody (mAb). Middle: DAPI. Right: Merge.

RNA pol II CTD phospho Ser2 antibody (mAb) tested by Western blot.

RNA pol II phospho Ser2 antibody (mAb) (Clone 3E7C7) tested by Western blot.
HeLa nuclear extract (30 µg per lane) probed with RNA pol II phospho Ser2 antibody (mAb) at a 1 µg/ml dilution.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA Pol II CTD phospho Ser2 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with RNA pol II CTD phospho Ser2 antibody (mAb).

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA pol II CTD phospho Ser2 antibody (mAb)
 

Data Thumbnails

RNA pol II CTD phospho Ser2 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of RNA pol II CTD phospho Ser2 mAb.
(Click image to enlarge and see details.)

RNA pol II CTD phospho Ser2 antibody (mAb) tested by Western blot.

Western blot of RNA pol II CTD phospho Ser2 mAb.
(Click image to enlarge and see details.)