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CID antibody (mAb)

Clone: 4F8
Aliases: CenH3, cid, BcDNA:RE21270, cenH3, CenH3/CID, CenH3[Cid], CenH3[CID], Cenp-A, CENP-A, CENP-A/Cid, CENP-A/CID, CENP-C, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID/CENP-A, Cid[Mel], Dmel\CG13329
商品コード: 61735 Format: 100 µg ¥67,000 Buy Now
商品コード: 61736 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:35 kDa
Reactivity:Drosophila

Applications

Immunofluorescence Validated Immunocytochemistry Validated

for CID antibody (mAb) (Clone 4F8)Application Notes


Published Applications:
ICC/IF
See reference for more information. Individual optimization may be required.

For an antibody against CID that works in Western blotting, use Catalog No. 39713.

for CID antibody (mAb) (Clone 4F8)Immunogen

This antibody was raised against a recombinant protein corresponding to the N-terminus of drosophila CID.

for CID antibody (mAb) (Clone 4F8)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CID antibody (mAb) (Clone 4F8)References

for CID antibody (mAb) (Clone 4F8)Background

CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CID antibody (mAb).

for CID antibody (mAb) (Clone 4F8)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CID antibody (mAb) (Clone 4F8)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CID antibody (mAb)