Histone H2A.Z.2.2 antibody (mAb)

RRID: AB_2793750
Clone: 1H11-11
Catalog No: 61737 Format: 100 µg ¥60,000 Buy
Catalog No: 61738 Format: 10 µg ¥21,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
12 kDa
Reactivity:
Human

Applications

Western Blot Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a peptide within the C-terminal region of human Histone H2A.Z.2.2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Bonisch, C., et al., 2012. "H2A.Z.2.2 is an alternatively spliced histone H2A.Z variant that causes severe nucleosome destabilization." Nucleic Acids Research 40(13): 5951-64.

Background

Histone H2A.Z (H2A Histone Family, Member V) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may serve to allow H2A.Z to function as an insulator of chromatin domains. In the case of H2A.Z.2.2, this variant has been shown to be responsible for destabilization of the nucleosome through the chaperone complexes TIP60 and SRCAP.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting