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SFMBT1 antibody (pAb)

Aliases: Scm-Like With Four Mbt Domains 1, Renal Ubiquitous Protein 1, Smr, Sfmbt
商品コード: 61759 Format: 100 µl ¥67,000 Buy Now
商品コード: 61760 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:110 kDa
Reactivity:Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

for SFMBT1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,500 dilution

Published Applications:
ChIP-Seq
ChIP
IF
IP
WB
See references for more information. Individual optimization may be required.

for SFMBT1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse SFMBT1.

for SFMBT1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SFMBT1 antibody (pAb)References

 
SFMBT1 antibody (pAb) tested by Western blot.

SFMBT1 antibody (pAb) tested by Western blot.
Detection of SFMBT1 antibody by Western blot. The analysis was performed using mouse testes nuclear extract (30 µg) and SFMBT1 antibody at a 1:1,000 dilution.

for SFMBT1 antibody (pAb)Background

SFMBT1 (Scm-Like With Four Mbt Domains 1) is a histone-binding protein, which is part of various corepressor complexes. Mediates the recruitment of corepressor complexes to target genes, followed by chromatin compaction and repression of transcription. Plays a role during myogenesis: required for the maintenance of undifferentiated states of myogenic progenitor cells via interaction with MYOD1. Interaction with MYOD1 leads to the recruitment of associated corepressors and silencing of MYOD1 target genes. Part of the SLC complex in germ cells, where it may play a role during spermatogenesis.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SFMBT1 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SFMBT1 antibody (pAb).

for SFMBT1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SFMBT1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SFMBT1 antibody (pAb)
 

Data Thumbnails

SFMBT1 antibody (pAb) tested by Western blot.

Western blot of SFMBT1 pAb.
(Click image to enlarge and see details.)