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Histone H1 antibody (mAb)

Clone: 1G6-E3
商品コード: 61785 Format: 100 µg ¥67,000 Buy Now
商品コード: 61786 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:35 kDa
Reactivity:Drosophila

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

for Histone H1 antibody (mAb) (Clone 1G6-E3)Application Notes


Validated Applications:
Western Blot: 0.2 - 1 µg/ml

Published Applications:
ChIP-Seq
ChIP
IP
IF
WB
See references for more information. Individual optimization may be required.

References
Iwasaki et. al., 2016, Molecular Cell 63, p. 408-419

for Histone H1 antibody (mAb) (Clone 1G6-E3)Immunogen

This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals; the antibody is not likely to cross-react to human, mouse or rat Histone H1. 

for Histone H1 antibody (mAb) (Clone 1G6-E3)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. 

 
Western Blot of Histone H1 antibody (clone 1G6-E3)

Histone H1 antibody tested by Western Blot.
Schneider’s L2 Drosophila cell lysate (cat. no. 36087) probed with Histone H1 antibody (0.5 µg/ml)

for Histone H1 antibody (mAb) (Clone 1G6-E3)Background

The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H1 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H1 antibody (mAb).

for Histone H1 antibody (mAb) (Clone 1G6-E3)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H1 antibody (mAb) (Clone 1G6-E3)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H1 antibody (mAb)
 

Data Thumbnails

Western Blot of Histone H1 antibody (clone 1G6-E3)

Western Blot of Histone H1 antibody (clone 1G6-E3)
(Click image to enlarge and see details)