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Histone H3 antibody (pAb)

商品コード: 61799 Format: 100 µg ¥69,000 Buy Now
商品コード: 61800 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Histone H3 antibody (pAb)Application Notes


Validated Applications:
ChIP-Seq: 4 µg per ChIP
WB: 0.1 – 1 µg/µl

for Histone H3 antibody (pAb)Immunogen

This antibody was raised against Recombinant Histone H3 from Xenopus laevis, which shares 99% identity to human H3 (134/136 aa are identical).

for Histone H3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
Histone H3 antibody (pAb) tested by ChIP-Seq.

Histone H3 antibody tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from Farage human lymph-node derived B cells and 4 µl Histone H3 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3 binding sites.

Histone H3 antibody (pAb) tested by Western blot.

Histone H3 antibody tested by Western blot
20 µg HeLa cell nuclear extract was run on an SDS-PAGE gel and probed with Histone H3 antibody (pAb) at 0.5 µg/ml.

MW: 17 kDa

for Histone H3 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 antibody (pAb).

for Histone H3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3 antibody (pAb)
 

Data Thumbnails

Histone H3 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3 pAb.
(Click image to enlarge and see details.)

Histone H3 antibody (pAb) tested by Western blot.

Western blot of Histone H3 pAb.
(Click image to enlarge and see details.)