Recombinant YEATS2 (200-340) protein

Catalog No: 81099 Format: 100 µg ¥94,000 Buy
Catalog No: 81899 Format: 1 mg ¥570,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

YEATS2 (YEATS Domain Containing 2), is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at ‘Lys-27’ (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.

Application Notes

Recombinant YEATS2 (200-340) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Binding Assay Conditions: 3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in a 10 µl reaction system containing 50mM HEPES-NaOH pH7.5, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

Protein Details

Recombinant human YEATS2 (200-340) that includes amino acids 200-340 (accession number NP_001338299.1) was expressed in E. coli cells with an N-terminal 6×His tag and a C-terminal Flag tag. The molecular weight of the protein is 21.5 kDa.

 

Recombinant YEATS2 (200-340) protein gel
12.5% SDS-PAGE Coomassie staining
MW: 21.5 kDa
Purity: >80%

Recombinant YEATS2 (200-340) protein activity assay
H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

Recombinant YEATS2 (200-340) protein activity assay
H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS2 (200-340) protein in reaction buffer for 1 hour, then FLAG antibody and SA-XL665 mixture was added to each reaction system for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. 

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.