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LightSwitch™ Synthetic Response Elements

the largest collection of synthetic response element reporters

LightSwitch™ Synthetic Response Element reporter constructs simplify the task of determining the effect that test compounds and/or conditions have on over 90 different transcription factors. Each synthRE vector contains an optimized synthetic response element, made up of repeats of a transcription factor binding site motif, cloned upstream of a minimal promoter and the RenSP luciferase gene in the Long-range Enhancer Reporter Vector, pLightSwitch_LR vector. These transfection-ready LightSwitch GoClones® together with the highly optimized assay reagents, transfection reagents, controls and detailed protocols allows you to perform your transcription factor reporter assay experiments immediately. A selection of these response elements are also available as validated pathway reporter stable cell lines.

If you can't find the exact response element or sequence you are looking for, our Custom Services can clone any sequence from the human, mouse or rat genomes, or mutagenize an existing GoClone® construct to fit your needs.

Applications

  • Use as a sensor in a primary screen to identify compounds or conditions that affect the activity of a biological pathway. Follow up with a secondary screen on the activity of multiple endogenous promoters from the pathway to detect subtle differences between compounds or conditions
  • Use as a positive control in your pathway-specific cell-based assays
  • Use as a highly sensitive response element for generating dose response curves

Note: All LightSwitch constructs MUST be used with the LightSwitch Luciferase Assay Reagents to obtain optimal results with our novel RenSP luciferase. We also recommend that you include appropriate positive & negative control vectors when you perform your assays.

 
Name Pathway Product Info Format Cat No. Price  
AARE synthRE Amino Acid Deprivation Get Info 5 µg 32101 ¥77,000 Buy
ATF-1 synthRE cAMP/PKA Get Info 5 µg 32110 ¥77,000 Buy
ATF-6 synthRE Estrogen Receptor Stress Response Get Info 5 µg 32111 ¥77,000 Buy
BMP synthRE v1 Bone Morphogenesis Get Info 5 µg 32112 ¥77,000 Buy
BMP synthRE v2 Bone Morphogenesis Get Info 5 µg 32113 ¥77,000 Buy
BMP synthRE v3 Bone Morphogenesis Get Info 5 µg 32114 ¥77,000 Buy
C/EBP alpha synthRE C/EBP Alpha Get Info 5 µg 32115 ¥77,000 Buy
C/EBP beta synthRE C/EBP Beta Get Info 5 µg 32116 ¥77,000 Buy
CREB synthRE cyclic AMP Get Info 5 µg 32117 ¥77,000 Buy
DBP synthRE Insulin Get Info 5 µg 32119 ¥77,000 Buy
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LightSwitch™ Synthetic Response Elements Data Sheet

Each Synthetic Response Element reporter construct includes an optimized synthetic response element that is made up of multiple repeats of a transcription factor binding site motif. In contrast to our LightSwitch Promoter Reporter vectors, which contain endogenous human promoters, Synthetic Response Element vectors contain the synthetic element cloned upstream of a minimal HSV-TK promoter and the RenSP luciferase reporter gene in the pLightSwitch_LR vector. This makes the empty pLightSwitch_LR vector ideal for use as a negative control, as it contains only a basal promoter. For complete information on the pLightSwitch_LR vector, please see the Empty LightSwitch™ Reporter Vectors page.

pLightSwitch_LR vector diagram

AR Synthetic Response Element (Androgen Receptor)

HT1080 fibrosarcoma cells were co-transfected with the AR synthRE reporter construct (Cat. No. 32107) and an AR cDNA construct according to our standard protocols. Half of the transfected cells were treated with 10 nM R1881 for 24 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch AR synthRE reporter construct

CREB Synthetic Response Element

HT1080 fibrosarcoma cells were transfected with the CREB synthRE reporter construct (Cat. No. 32117) according to our standard protocols. Half of the transfected cells were treated with 100 nM PMA for 4 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch CREB synthRE reporter construct

ER Synthetic Response Element (Estrogen Receptor)

HT1080 fibrosarcoma cells were co-transfected with the ER synthRE reporter construct (Cat. No. 32125) and an ER cDNA construct according to our standard protocols. Half of the transfected cells were treated with 10 nM β-estradiol for 24 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch ER synthRE reporter construct

GR Synthetic Response Element (Glucocorticoid Receptor)

HT1080 fibrosarcoma cells were transfected with the GR synthRE reporter construct (Cat. No. 32140) according to our standard protocols. Half of the transfected cells were treated with 100 nM dexamethasone (DMSO) for 4 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch GR synthRE reporter construct

HIF-1 alpha Synthetic Response Element (Hypoxia)

HT1080 fibrosarcoma cells were transfected with the HIF-1 alpha synthRE reporter construct (Cat. No. 32141) according to our standard protocols. Half of the transfected cells were treated with a low oxygen condition (1% O2) for 24 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch HIF-1 alpha synthRE reporter construct

HSF Synthetic Response Element (Heat Shock)

HT1080 fibrosarcoma cells were transfected with the HSF synthRE reporter construct (Cat. No. 32144) according to our standard protocols. The transfected cells were then incubated at 37°C or 43°C for 8 hours. The cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch HSF synthRE reporter construct

NFκB Synthetic Response Element (Inflammation)

HT1080 fibrosarcoma cells were transfected with the NFκB synthRE reporter construct (Cat. No. 32160) according to our standard protocols. Half of the transfected cells were treated with 50 ng/ml TNF-α for 8 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch NFkB synthRE reporter construct

STAT1 Synthetic Response Element (Inflammation)

HT1080 fibrosarcoma cells were transfected with the STAT1 synthRE reporter construct (Cat. No. 32183) according to our standard protocols. Half of the transfected cells were treated with 500 U/ml Interferon-α for 8 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch STAT1 synthRE reporter construct