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Recombinant PKA protein

商品コード: 31158 Format: 50 µg
Expressed In: E. coli Protein Species: Human

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for Recombinant PKA proteinContents

This product has been discontinued

50 µg of Recombinant PKA protein in 30 mM K-phosphate, 150 mM KCl, 1 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.4.

for Recombinant PKA proteinApplication Notes

Recombinant PKA is suitable for kinase assays. The molecular weight of the protein is ~42.301 kDa. The activity of the protein is ~ 1,600,000 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to PKA peptide (LRRASLG) per minute at 30°C. Recommended Kinase Reaction Buffer: 20 mM Tris-HCL, 25 mM β-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate and 1 mM DTT, pH 7.5. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant PKA proteinProtein Details

Recombinant human PKA protein was expressed in E. coli with an N-terminal His-Tag and is suitable for labeling PKA substrates. The accession number is NM_002730. Purified by Ni-agarose chromatography to > 95% by SDS-PAGE.

for Recombinant PKA proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant PKA protein