Recombinant Histone H3K27ac

Catalog No: 31290 Format: 25 µg
Expressed In: Synthetic Protein Species: Human

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Contents

25 µg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Application Notes

This product has been discontinued:
Please refer to our alternative product Catalog No. 81049

Protein Details

Recombinant Histone H3K27ac proteins are synthetic modified histones containing acetylation at K27. The Recombinant Histone H3K27ac proteins correspond to the native histone sequence and do not contain any amino acid substitutions or residue analogs. Protein concentration was determined using Bradford assay. The recombinant histone is >95% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,313 Daltons.

References


This product was used in the following publications:
Nat. Immunol. (2016). 17(6):695-703. PMID: 27111144. (HDAC Assay)

 

SDS-PAGE gel analysis of Recombinant Histone H3K27ac protein.

Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.