PC-3 nuclear extract

Catalog No: 36082 Format: 200 µg ¥32,000 Buy

Contents

200 µg of PC-3 nuclear extract at 6.88 µg/µl.

Background

PC-3 nuclear extract was prepared from cell cultures of the human prostate adenocarcinoma PC-3 cell line. The PC-3 cell line was derived from an advanced androgen-independent bone metastasis of a 64 year old Caucasian male patient with Grade IV prostatic cancer. PC-3 cells are considered the 'classical' prostate tumor cell line and are characterized by a high metastatic potential, the expression of Prostate Specific Antigen (PSA), and low acid phosphatase and testosterone-5-α reductase activity. PC-3 cells are most commonly used in research as a model of prostate cancer progression.

Application Notes

PC-3 nuclear extract is specifically recommended for studies related to prostate cancer.

Extract Origin

Human prostate adenocarcinoma

Extract Composition

PC-3 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.