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Lamin B1 antibody (pAb)
| RRID: AB_2615027 |
| Aliases: LMNB1, LMN, ADLD, LMN2, LMNB |
| Catalog No: 39095 | Format: 100 µl | Discontinued | |
Applications
Application Notes
This product has been discontinued:
Validated Applications:
WB: 1:100 - 1:500 dilution
Published Applications: see references
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
Immunogen
This Lamin B1 antibody was raised against a full-length protein corresponding to human Lamin B1.
Buffer
Rabbit serum containing 0.1% sodium azide. Sodium azide is highly toxic.
Lamin B1 antibody tested by Western blot.
Lamin B1 detection by Western blot analysis. Lamin B1 is detected in HeLa nuclear extracts using Lamin B1 rabbit polyclonal antibody at a 1:100 (lane 1) and a 1:500 (lane 2) dilution.
Background
Lamin B1 (LMN, ADLD), along with the A and C Lamins are involved in maintaining the spatial organization of nuclear compartments. Depletion of Lamin B1 results in the disruption of RNA synthesis by RNA pol II, and eventually migration of chromatin to the nuclear periphery and dispersion of the nucleoli. The role of Lamin B1 in maintaining the nucleoskeleton may be to bind to nucleophosmin/B23.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Lamin B1 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
