Active Motif,
Tools to analyze nuclear function,
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Hbo1 antibody (pAb)

Aliases: KAT7, HBOA, MYST2, ZC2HC7
商品コード: 39105 Format: 200 µl ¥68,400 Buy Now
商品コード: 39106 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Molecular Weight:75 kDa
Reactivity:Human, Other (Wide Range)


Western Blot Validated

for Hbo1 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts. 

for Hbo1 antibody (pAb)Immunogen

This Hbo1 antibody was raised against a synthetic peptide containing amino acids 158-172 of human Hbo1.

for Hbo1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Hbo1 antibody (pAb)References

Hbo1 antibody (pAb) tested by Western blot.

Hbo1 pAb tested by Western blot.
Detection of Hbo1 by Western blot analysis. HeLa whole-cell extract (15 µg) was probed with Hbo1 pAb at a 1:500 dilution.

for Hbo1 antibody (pAb)Background

Hbo1 (HAT binding to ORC1) is a MYST-family histone acetyltransferase (HAT) that forms a complex with ING4/5, JADE1/2/3, and Eaf6, which is found enriched on coding regions of genes, supporting a role in transcriptional elongation. In addition, Hbo1 is thought to participate in events controlling the initiation of DNA replication.

for Hbo1 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Hbo1 antibody.

for Hbo1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Hbo1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Hbo1 antibody (pAb)

Data Thumbnails

Hbo1 antibody (pAb) tested by Western blot.

Western blot of Hbo1 pAb.
(Click image to enlarge and see details.)