Mi-2 beta antibody (pAb)

RRID: AB_2614937
Aliases: CHD4, Mi-2b
Catalog No: 39289 Format: 100 µl ¥70,000 Buy
Catalog No: 39290 Format: 10 µl ¥20,000 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
240 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
IHC (FFPE): 1:100
ICC/IF: 1:500 - 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution

Published Applications:
ICC/IF
IHC
See references for more information. Individual optimization may be required.

Immunogen

This Mi-2B antibody was raised against a recombinant protein corresponding to amino acids 22-155 of mouse Mi-2 beta.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

Mi-2 beta antibody tested by immunofluorescence.
Top left: HeLa cells stained with Mi-2 beta antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

Mi-2 beta antibody tested by Western blot.
Whole-cell extract of mouse splenocytes stained with Mi-2 beta antibody (1:1,1000).

Mi-2 beta antibody (pAb) tested by Immunohistochemistry
Nuclear and cytoplasmic staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human skeletal muscle. Top Panel: Mi-2 beta antibody at 1:100 dilution. Bottom Panel: No primary antibody (2nd step antibody alone)

Background

Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Mi-2 beta antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting