Kaiso antibody (mAb)

RRID: AB_2793229
Clone: 6F1D
Aliases: ZBTB33, AW260253, Znf-kaiso, E130014G12Rik
Catalog No: 39365 Format: 200 µg ¥70,000 Buy
Catalog No: 39366 Format: 10 µg ¥20,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
95 kDa
Reactivity:
Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 0.5 - 4 µg/ml dilution

Immunogen

This Kaiso antibody was raised against a His-Tagged fusion protein corresponding to amino acids 103-499 of mouse Kaiso.

Buffer

PBS containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

Kaiso mAb (Clone 6F1D) tested by Western blot.
Nuclear extract of CSML0 cells (20 µg) was probed with Kaiso mAb (Clone 6F1D) (1 µg/ml dilution).

Background

Kaiso (Clone 6F1D) is a zinc-finger transcription factor protein that binds to methylated and non-methylated GTGCNA motifs, and is also known as ZNF-Kaiso or ZBTB33. Kaiso interacts with the cell adhesion cofactor p120 catenin and may also recruit N-CoR repressor complex to promote histone deacetylation and repress transcriptional activity.

Positive Control

Nuclear extracts form CSML0 cells can be used as a positive control for Kaiso antibody (Clone 6F1D).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting