L3MBTL2 antibody (pAb)
RRID: AB_2615062 |
Aliases: L3MBT, H-l(3)mbt-l |
Catalog No: 39569 | Format: 100 µl | ¥86,000 | Buy |
Catalog No: 39570 | Format: 10 µl | ¥24,000 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-qPCR, ChIP-Seq
IP
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This L3MBTL2 antibody was raised against a His-Tagged fusion protein containing amino acids 1-230 of human L3MBTL2.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Background
L3MBTL2 (Lethal (3) malignant brain tumor-like 2 protein) is a putative member of the polycomb group family and is the human homolog of Drosophila Sfmbt, critical for Hox gene silencing. L3MBTL2 contains one FCS-type zinc finger and four MBT (malignant brain tumor) repeats. L3MBTL2 associates with chromatin-remodeling complexes and helps inhibit the expression of proteins that trigger the cells into mitosis. L3MBTL2 is a part of a complex involved in the G0 phase of the cell cycle. This complex contains other proteins such as E2F-6, Max or HP1 gamma, and participates in transcriptional repression.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for L3MBTL2 pAb.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting