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SNF5 antibody (pAb)

Aliases: SMARCB1 SWI/SNF, RDT, INI1, Snr1, BAF47, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1
Catalog No: 39775 Format: 100 µl ¥68,400 Buy Now
Catalog No: 39776 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:60 kDa


Western Blot Validated

for SNF5 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for SNF5 antibody (pAb)Immunogen

This SNF5 antibody was raised against a peptide sequence)(CDAEMEKKIRDQDRNTR in the C-terminus of SNF5.

for SNF5 antibody (pAb)Buffer

Purified rabbit IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SNF5 antibody (pAb)References

SNF5 antibody (pAb) tested by Western blot.

SNF5 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of Jurkat cells probed with SNF5 antibody (pAb) at a dilution of 1:2,000.

for SNF5 antibody (pAb)Background

SNF5 (Sucrose Non Fermenting 5, also called INI1, BAF47 and SMARCB1) is a tumor suppressor protein that is a component of the SWI/SNF chromatin-remodeling complex that regulates gene expression through alterations in chromatin structure. Alterations in chromatin structure facilitated by ATP-dependant chromatin remodeling machines are thought to enhance recognition and binding of trans-acting factors that bind DNA and regulate transcription. SNF5 signals through the p16INK4a pathway to negatively regulate cell cycle progression and mediate chromosome stability. Mutations in SNF5 are a cause of malignant rhabdoid tumors in children.

for SNF5 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for SNF5 antibody.

for SNF5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SNF5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

SNF5 antibody (pAb)

Data Thumbnails

SNF5 antibody (pAb) tested by Western blot.

Western blot of SNF5 pAb.
(Click image to enlarge and see details.)