Active Motif,
Tools to analyze nuclear function,
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Ubn1 antibody (pAb)

Aliases: VT, VT4
商品コード: 39869 Format: 100 µl ¥68,400 Buy Now
商品コード: 39870 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:145 kDa


Western Blot Validated

for Ubn1 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for Ubn1 antibody (pAb)Immunogen

This Ubinuclein antibody was raised against a recombinant protein corresponding to amino acids 1-345 of human Ubn1.

for Ubn1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Ubn1 antibody (pAb)References

Ubn1 antibody (pAb) tested by Western blot.

Ubn1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 μg) probed with Ubn1 antibody at a dilution of 1:500.

for Ubn1 antibody (pAb)Background

UBN1 (Ubinuclein 1, also known as HIRA binding protein) is a ubiquitously expressed nuclear protein that acts as a novel regulator of senescence. UBN1 is involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. It is expressed in a number of cancer cell lines and tumors and may be required for replication-independent chromatin assembly. UBN1 is part of a large multi-subunit protein complex that interacts with CEBPA and Epstein-Barr virus BLZF1. It co-localizes with HIRA, PML and SP100 in the PML bodies of senescent cells. When overexpressed, UBN1 accumulates in the nucleus of cells defective for cytokinesis.

for Ubn1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Ubn1 antibody.

for Ubn1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Ubn1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Ubn1 antibody (pAb)

Data Thumbnails

Ubn1 antibody (pAb) tested by Western blot.

Western blot of Ubn1 pAb.
(Click image to enlarge and see details.)