DNMT3L antibody (pAb)

RRID: AB_2793390
Catalog No: 39907 Format: 100 µl ¥70,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This DNMT1 antibody was raised against a recombinant protein corresponding to full-length human DNMT3L.

Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

References

 

DNMT3L antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of human testicular cells probed with DNMT3L antibody at a dilution of 1:500.

Background

DNMT3L (DNA methyltransferase 3-like) is a nuclear protein with similarity to the DNMT family of proteins, but it does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation. DNMT3L is expressed in the presumptive gametes in mammalian embryos and is required for the establishment of genomic imprinting. DNMT3L has a PHD-like domain that allows binding to chromatin through interactions with the amino terminal tail of histone H3 that is devoid of lysine 4 methylation.

Positive Control

Active Motif's Human Testes nuclear extract (Catalog No. 36047) can be used as a positive control for DNMT3L antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting