CREB antibody (mAb)

RRID: AB_2614962
Clone: 82B514
Aliases: CREB1
Catalog No: 40961 Format: 100 µg ¥70,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
43 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This CREB antibody was raised against a synthetic peptide corresponding to human CREB.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 

CREB mAb tested by Western blot.
Detection of CREB by Western blot analysis. A protein band of approximate molecular weight of 43 kDa was detected using HeLa whole-cell extract and CREB mAb at a dilution of 2 µg/ml.

Background

CREB (cAMP response element binding) transcription factor binds as a homodimer to specific DNA sequences called cAMP response elements (CRE) and regulates transcription. CREB belongs to the leucine zipper family of DNA binding proteins. CREB induces transcription in response to hormonal stimulation.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for CREB antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting