DAP-3 antibody (mAb)

RRID: AB_2793460
Clone: 42C617
Aliases: DAP3, MRPS29, MRP-S29, bMRP-10
Catalog No: 40963 Format: 100 µg ¥86,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
45 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This DAP-3 antibody was raised against a synthetic peptide corresponding to amino acid residues 51-66 of human DAP-3.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

DAP-3 mAb tested by Western blot.
DAP-3 detection by Western blot. The analysis was performed using 10 µg Jurkat whole-cell extract and the DAP-3 mAb at a 1 µg/ml dilution.

Background

DAP-3 (Death Associated Protein 3) potentially binds ATP/GTP and may be a functional partner of the mitoribosomal protein S27. The DAP-3 gene encodes a 28S subunit protein that also participates in apoptotic pathways that are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon.

Positive Control

Active Motif's Jurkat cytoplasmic extract (Catalog No. 36402) can be used as a positive control for DAP-3 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting