ATF-2 phospho Thr71 antibody (mAb)

RRID: AB_2793467
Clone: 103C411
Aliases: HB16, CREB2, TREB7, CREB-2, CRE-BP1
Catalog No: 40975 Format: 100 µg Discontinued

Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
61 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

This product has been discontinued:
Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This ATF-2 phospho Thr71 antibody was raised against a synthetic peptide containing phosphorylated threonine at position 71 corresponding to human Phosphorylated-ATF-2.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

ATF-2 phospho Thr71 mAb tested by Western blot.
ATF-2 (phospho) detection by Western blot. Detection of ATF-2 phosphorylation in HeLa cell extract with ATF-2 phospho Thr71 mAb.
    Lane A: Untreated HeLa nuclear extract;
    Lane B: HeLa nuclear extract treated with TNF-α for 1 hour.

Background

ATF-2 (activating transcription factor 2) transcription factor is a member of the leucine-zipper family of proteins and was previously known as cAMP responsive element binding protein 2 (or CREB2). ATF 2 is a transcriptional activator that binds the cAMP-responsive element (CRE) and interacts with c-Jun to stimulate CRE transcription. This protein is also capable of acetylating Histone H2B and Histone H4 in vitro as a histone acetyltransferase (HAT).

Positive Control

Active Motif's HeLa nuclear extract (TNF-α stimulated) (Catalog No. 40210) can be used as a positive control for ATF-2 phospho Thr71 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting