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AEBP2 antibody (pAb)

商品コード: 61539 Format: 100 µl ¥68,400 Buy Now
商品コード: 61540 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:52 kDa


Western Blot Validated

for AEBP2 antibody (pAb)Application Notes

Validated Applications:
WB: 1:1,000 - 1:5,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for AEBP2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse AEBP2.

for AEBP2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for AEBP2 antibody (pAb)References

AEBP2 antibody (pAb) tested by Western blot.

AEBP2 antibody (pAb) tested by Western blot.
AEBP2 detection by Western blot. The analysis was performed using 20 µg P19 nuclear cell extract and AEBP2 antibody at a 1:2,500 dilution. An unspecified band is detected around 160 kDa.

for AEBP2 antibody (pAb)Background

AEBP2 (AE binding protein 2) is a DNA-binding transcriptional repressor and component of the PRC2 complex, which methylates 'Lys-27' residues of Histone H3.

for AEBP2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AEBP2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AEBP2 antibody (pAb)

Data Thumbnails

AEBP2 antibody (pAb) tested by Western blot.

Western blot of AEBP2 pAb.
(Click image to enlarge and see details.)