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TRIM24 antibody (pAb)

Aliases: PTC6, TF1A, TIF1, RNF82, TIF1A, hTIF1, TIF1ALPHA
商品コード: 61551 Format: 100 µl ¥68,400 Buy Now
商品コード: 61552 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:140 kDa


Western Blot Validated Immunoprecipitation Validated

for TRIM24 antibody (pAb)Application Notes

Validated Applications:
IP: 5 - 10 µl per IP
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for TRIM24 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human TRIM24.

for TRIM24 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TRIM24 antibody (pAb)References

TRIM24 antibody (pAb) tested by immunoprecipitation.

TRIM24 antibody (pAb) tested by Immunoprecipitation.
10 µl of TRIM24 antibody was used to immunoprecipitate TRIM24 from 200 µg of HEK293 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the TRIM24 antibody at a dilution of 1:1,000.

TRIM24 antibody (pAb) tested by Western blot.

TRIM24 antibody (pAb) tested by Western blot.
Nuclear extract of HEK293 cells (30 µg) probed with TRIM24 antibody (pAb) at a dilution of 1:1,000.

for TRIM24 antibody (pAb)Background

TRIM24 (tripartite motif containing 24) is a transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).

for TRIM24 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TRIM24 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

TRIM24 antibody (pAb)

Data Thumbnails

TRIM24 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of TRIM24 pAb.
(Click image to enlarge and see details.)

TRIM24 antibody (pAb) tested by Western blot.

Western blot of TRIM24 pAb.
(Click image to enlarge and see details.)