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Histone H3S28ph antibody (mAb)

Clone: 5F9A9
商品コード: 61695 Format: 100 µg ¥68,400 Buy Now
商品コード: 61696 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Monoclonal
Purification:Protein A Chromatography
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)


Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)Application Notes

Validated Applications:
ICC/IF: 2 - 10 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)Immunogen

This antibody was raised against a peptide corresponding to phospho-serine 28 of human Histone H3.

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)References

Histone H3S28ph antibody (mAb) tested by immunofluorescence.

Histone H3S28ph antibody (mAb) (Clone 5F9A9) tested by Immunofluorescence.
Left: Formaldehyde fixed mitotic DM4 cell stained with Histone H3S28ph antibody (mAb).
Right: Hoechst stain.

Histone H3S28ph antibody (mAb) tested by Western blot.

Histone H3S28ph antibody (mAb) (Clone 5F9A9) tested by Western blot.
Detection of Histone H3S28ph antibody by Western blot. The analysis was performed using 20 µg of untreated (lane 1) or colcemid treated (lane 2) HeLa nuclear extract with Histone H3S28ph antibody at a 1 µg/ml dilution.

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3S28ph antibody (mAb).

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3S28ph antibody (mAb) (Clone 5F9A9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Histone H3S28ph antibody (mAb)

Data Thumbnails

Histone H3S28ph antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3S28ph mAb.
(Click image to enlarge and see details.)

Histone H3S28ph antibody (mAb) tested by Western blot.

Western blot of Histone H3S28ph mAb.
(Click image to enlarge and see details.)