Histone H2A.J antibody (pAb)

RRID: AB_2793769
Catalog No: 61793 Format: 100 µl ¥86,000 Buy
Catalog No: 61794 Format: 10 µl ¥24,000 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Reactivity:
Human, Mouse

Applications

Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:2500 dilution
DB: 1:1000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
Histone H2A.J antibody (pAb) tested by Western blot.

Western blot of Histone H2A.J pAb.
20 µg Nuclear Extract of HeLa cells (Lane 1) or 40 µg mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3).
MW:14kD

Histone H2A.J antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2A.J pAb.
Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.

Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting