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Recombinant MLLT3 / AF9 (1-138) protein, His/FLAG Tag
| Catalog No: 81192 | Format: 20 µg | ¥88,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
MLLT3 (Myeloid/Lymphoid Or Mixed-Lineage Leukemia; translocated To, 3), also known as AF9 or-YEATS3, is a component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.
Application Notes
Recombinant MLLT3 / AF9 (1-138) protein, His / FLAG tag is suitable for use in binding assay, inhibitor screening, and selectivity profiling.
Protein Details
Recombinant human MLLT3 / AF9 (1-138) protein, His / FLAG-Tag that includes amino acids 1-138 (accession number NP_004520.2) was expressed in E. coli cells with an N-terminal 6xHis Tag and a C-terminal FLAG-Tag. The molecular weight of the protein is 20.8 kDa.
Recombinant MLLT3 / AF9 (1-138) protein, His
/ FLAG-Tag
10% SDS-PAGE Coomassie staining
MW: 20.8 kDa
Purity: >79%
Recombinant MLLT3 / AF9 (1-138) protein, His / FLAG-Tag activity assay
3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 µl system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.
Recombinant MLLT3 / AF9 (1-138) protein, His / FLAG-Tag activity assay
3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 µl system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
