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AbFlex® MAZ antibody (rAb)

Aliases: MYC Associated Zinc Finger Protein, Transcription Factor Zif87, ZNF801
Catalog No: 91205 Format: 100 µg ¥62,000 Buy Now
Catalog No: 91206 Format: 10 µg ¥17,000 Buy Now

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Antibody Type:Recombinant
Purification:Protein A Chromatography
Molecular Weight:55 kDa


Western Blot Validated

for AbFlex MAZ antibody (rAb)Application Notes

Validated Applications:
WB: 1 - 4 µg/ml dilution.

Note: For optimal results, primary antibody incubations should be performed
at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions
may also reduce background. Individual optimization may be required.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39935.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for AbFlex MAZ antibody (rAb)Immunogen

This antibody was raised against human Maz protein [MYC-associated zinc finger protein], NP_002374.

for AbFlex MAZ antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

for AbFlex MAZ antibody (rAb)References

AbFlex<sup>®</sup> MAZ antibody (rAb) tested by Western blot.

AbFlex® MAZ antibody (rAb) tested by Western Blot.
10 µg MCF7 cell nuclear extract was run on SDS-PAGE, and probed with 2 µg/ml AbFlex MAZ antibody. MW: 55 kDa

for AbFlex MAZ antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.  MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells.

for AbFlex MAZ antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex MAZ antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AbFlex® MAZ antibody (rAb)

Data Thumbnails

AbFlex<sup>®</sup> MAZ antibody (rAb) tested by Western blot.

Western blot of AbFlex® MAZ antibody (rAb).
(Click image to enlarge and see details.)