pLightSwitch_Prom Base pairs 3656 Multiple cloning region 1-72 RenSP reporter gene (synthetic renilla luciferase; includes mODC PEST) 112-1170 SV40 late poly(A) region 1201-1422 ColE1-derived plasmid replication origin 1747 Synthetic Beta-lactamase (Ampr) coding region 2538-3398 Synthetic poly(A) signal/transcriptional pause site 3503-3656 Forward Sequencing Primer: TCCATCAAAACAAAACGAAACAA Reverse Sequencing Primer: AGTCGAGCACGTTCATCTGCTT GGCCTAACTGGCCGGTACCTGAGCTCTTACGCGTGCTAGCCCGGGCTCGAGATCTGCGAT CTAAGTAAGCTTAACTAAGTAAGGCATTCCGGTACTGTTGGTAAAGCCACCATGGCTTCC AAGGTGTACGACCCGGAGCAGCGCAAGAGGATGATCACCGGCCCTCAGTGGTGGGCTCGG TGCAAGCAGATGAACGTGCTCGACTCCTTCATCAACTACTACGACAGCGAGAAACATGCG GAGAACGCCGTGATCTTCCTCCACGGCAACGCCGCTTCCTCCTACCTGTGGCGCCACGTC GTGCCCCACATCGAGCCCGTCGCCCGGTGCATCATCCCTGATCTGATCGGGATGGGGAAG AGCGGGAAGAGCGGCAACGGCAGCTACCGCCTGCTCGACCACTACAAGTACCTCACCGCC TGGTTCGAGCTGCTGAACCTCCCCAAGAAGATCATCTTTGTGGGCCACGACTGGGGCGCT TGTCTCGCTTTTCACTACTCCTACGAGCACCAGGATAAGATCAAGGCTATCGTGCATGCT GAGAGCGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCCGATATCGAGGAGGATATT GCTCTGATCAAGTCCGAGGAGGGCGAGAAGATGGTCCTGGAGAATAACTTCTTCGTGGAG ACTATGCTGCCTAGCAAGATCATGCGCAAGCTGGAGCCCGAGGAGTTCGCTGCTTACCTG GAGCCCTTCAAGGAGAAGGGCGAGGTCAGAAGACCAACCCTCAGCTGGCCTCGGGAGATC CCTCTGGTCAAGGGCGGGAAGCCGGACGTGGTGCAGATCGTCCGGAACTACAACGCCTAC CTGCGCGCCAGCGACGACCTGCCTAAGATGTTCATCGAGTCCGACCCCGGCTTCTTCAGC AACGCTATCGTGGAGGGCGCCAAGAAGTTCCCCAACACCGAGTTCGTGAAGGTGAAGGGC CTCCACTTCTCCCAAGAGGACGCCCCTGATGAGATGGGGAAGTACATCAAGAGCTTCGTC GAGCGCGTCCTCAAGAACGAGCAGAATTCTCACGGCTTCCCTCCCGAGGTGGAGGAGCAG GCCGCCGGCACCCTGCCCATGAGCTGCGCCCAGGAGAGCGGCATGGATAGACACCCTGCT GCTTGCGCCAGCGCCAGGATCAACGTCTAATCTAGAGTCGGGGCGGCCGGCCGCTTCGAG CAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAA AATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCA ATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGT GGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGATAAGGATCCGT CGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATG ACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCG GCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC AGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAG TCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGC AGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA GTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAA GCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG TAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGG GATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCGGCCGCAAATG CTAAACCACTGCAGTGGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGT CTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAG GGCTTACCATCTGGCCCCAGCGCTGCGATGATACCGCGAGAACCACGCTCACCGGCTCCG GATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACT TTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCA GTTAATAGTTTGCGCAACGTTGTTGCCATCGCTACAGGCATCGTGGTGTCACGCTCGTCG TTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCC ATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTG GCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCA TCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGT ATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGC AGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATC TTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCA TCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAA AAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCGTCCTTTTTCAATATTAT TGAAGCATTTATCAGGGTTACTAGTACGTCTCTCAAGGATAAGTAAGTAATATTAAGGTA CGGGAGGTATTGGACAGGCCGCAATAAAATATCTTTATTTTCATTACATCTGTGTGTTGG TTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAACAAAACGAAACAAAA CAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCT