Products for Epigenetics, Chromatin Biology
& Transcriptional Regulation

At Active Motif, we develop innovative cell biology-based research tools and biocomputing resources that help researchers elucidate the function, regulation and interactions of the genes and their encoded proteins. Our goal is to provide our customers with high-quality products complemented by superior service & support.

New & Notable Products

• New 5-Methylcytosine polyclonal antibody

  Active Motif now offers a polyclonal version of our existing 5‑Methylcytosine mAb antibody for use in MeDIP and Dot Blot analysis.

• 5-Carboxylcytosine DNA Standard Kit is now available

  Active Motif understands the importance of using appropriate controls to confirm experimental results. We are pleased to announce the release of our 5-Carboxylcytosine DNA Standard Kit which includes double-stranded DNA oligonucleotides containing the 5-carboxylcytosine DNA modification as well as an unmodified DNA control. More >

• Now offering ChIP-IT^® Control Kits for qPCR

  Active Motif now offers ChIP-IT^® Control qPCR Kits for the analysis of human, mouse or rat chromatin samples. These kits will confirm the chromatin preparation and IP procedure worked properly and allow you to confirm the quality of your test antibody and qPCR primer sets. The ChIP-IT Control qPCR Kits should be used in combination with Active Motif's ChIP-IT Express or ChIP-IT Express Enzymatic Kits. For more details on all of our ChIP products, please click here.

• ChIP-Seq & ChIP-chip Antibodies Available

  For scientists requiring validated antibodies for use in ChIP-Seq and ChIP-on-chip, Active Motif is now in the process of re-evaluating its large selection of histone modifying and transcription factor antibodies. ChIP-Seq/ChIP-chip Antibody List.

• Phosphatase & Protease Inhibitor Cocktails

  Active Motif now sells Phosphatase Inhibitor Cocktail and Protease Inhibitor Cocktail to improve your nuclear, cytoplasmic and whole-cell extraction stability.

• New Antibodies for 5-formylcytosine & 5-carboxylcytosine

  DNA methylation is an important part of mechanisms regulating genome accessibility and function, and Active Motif offers a number of kits and reagents to study the different forms of methylated DNA. In addition to 5-methylcytosine and 5-hydroxymethylcytosine methylation, we now offer highly specific and well validated antibodies for the study of two novel forms of methylation, 5-formylcytosine and 5‑carboxylcytosine.

• New EpiShear™ Multi-Sample Sonicator

  The new EpiShear™ Multi-Sample Sonicator is a high-intensity cup horn sonicator that can shear up to eight vials (20 µl - 1.2 ml sample per vial) of chromatin or DNA simultaneously for use in chromatin immunoprecipitation (ChIP), DNA methylation studies and Next-Gen sequencing. More >

• EpiShear™ Cooled Sonication Platform Improves Reproducibility

  The new EpiShear™ Cooled Sonication Platform improves sample-to-sample reproducibility when using probe sonicators by keeping the sample cold while enabling the probe to be positioned at the same depth in the sample every time. More >

• New Hydroxymethyl Collector™ Kit for Biotin-based 5-hmC Capture

  Active Motif continues to expand its portfolio of products to study 5-hydroxymethylcytosine. Our new Hydroxymethyl Collector™ Kit is able to chemically label 5-hmC residues for specific enrichment of DNA fragments containing 5-hydroxymethylcytosine. A biotin-streptavidin capture method is used to isolate the labeled 5-hmC residues. More >

• Dounce Homogenizer for Better Chromatin and Lysate Preparation

  To help improve the quality of your sample preparations for chromatin immunoprecipitation experiments or for regular extraction of cell or tissue lysates, Active Motif now offers a Dounce Homogenizer. There are two sizes available for either small or large scale sample preparations. More >

• Validated qPCR Primer Sets for use as ChIP Controls

  Active Motif offers a large variety of human- and mouse-specific ChIP Control qPCR Primer Sets for many of the more common ChIP targets. These positive and negative controls have been validated and proven to work in both qPCR and endpoint PCR, and are used regularly by our Epigenetic Services division. More >

• MeDIP Kit Now Available for 5-methylcytosine DNA Enrichment

  Active Motif now offers a MeDIP Kit for the isolation of single-stranded DNA containing 5-methylcytosine. This kit can be used alone, or in combination with our hMeDIP Kit. More >

• New hMeDIP Kit for Specific Enrichment of 5-hmC DNA

  To better understand the role of 5-hydroxymethylcytosine (5-hmC) DNA methylation, Active Motif introduces its hMeDIP Kit, which specifically enriches for 5-hmC DNA fragments. Unlike traditional MeDIP, the hMeDIP Kit can be used with double-stranded DNA, which greatly simplifies downstream analysis. More >

• Get Analysis-ready DNA with the Chromatin IP DNA Purification Kit

  Quickly and easily clean up your ChIP DNA with the Chromatin IP DNA Purification Kit. Remove contaminants that interfere with downstream analysis (microarray, qPCR, Next-Gen sequencing). The kit can also prepare DNA for use with our hMeDIP, MethylCollector™ Ultra and UnMethylCollector™ Kits.

• Methylated DNA Standard Kit for the Study of DNA Methylation

  Try our new Methylated DNA Standard Kit for experiments examining the different types of DNA methylation. It includes DNA derived from the APC promoter that has no methylation, 5-methylcytosine methylation or 5-hydroxymethylcytosine methylation. Also included are specific primers to aid in detection by PCR.