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Histone Acetyltransferase Assay Kit (Fluorescent)

screen inhibitors or calculate HAT activity

The HAT Assay Kit is a fluorescent method to assay samples for histone acetyltransferase (HAT) activity or to screen HAT inhibitors. The assay is easy to use, features a simple protocol that gives you results in 30 minutes and is ideal for high-throughput usage in 96-well plates.

 
Name Format Cat No. 価格 (税抜)  
Recombinant GCN5 protein 5 µg 31204 ¥72,000 Buy Now
Recombinant p300 protein, catalytic domain 5 µg 31205 ¥72,000 Buy Now
HAT Assay Kit (Fluorescent) 1 x 96 rxns 56100 ¥83,000 Buy Now

HAT Assay Kitには96ウェルプレートで阻害物質をスクリーニングを行う際に十分量のリコンビナントタンパク質p300, HATの阻害物質のコントロールとしてAnacardic acidが付属しています。キット内のバッファーは全て, HAT活性測定や阻害物質スクリーニングにおいて干渉がないことを確認しています。またHATの基質としてHistone H3およびHistone H4の各ペプチドが含まれています。

 
 
 
図1: p300 p300 タンパク質の活性と阻害

p300タンパク質 50 ngのHAT活性を50 µM acetyl-CoA および基質となる 50 µM Histone H3 またはHistone H4ペプチドを用いて解析した。HAT活性は 15 µM anacardic acid または 25 µM garcinolで阻害された。

Contents & Storage

1 x 96 rxns of recombinant p300 protein, catalytic domain; histone H3 and H4 substrate peptides, Acetyl-CoA, 5X Assay Buffer, Developer and Developing & Stop Solutions. A 96-well plate and plate sealer are also included. Recombinant p300 protein, catalytic domain; and Acetyl-CoA are to be stored at -80°C. Store histone H3 and H4 substrate peptides, 5X Assay Buffer, Developer and Developing Solution at -20°C. Stop Solution, the 96-well plate and plate sealer may be stored at room temperature. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.