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抗体

AbFlex® リコンビナント抗体

高い特異性かつ再現性をもつ抗体

AbFlex抗体は,非常に特異的かつ再現性がある抗体を産生するために規定されたDNA配列を用いて生成されたリコンビナント抗体(rAb)です。 AbFlex抗体のユニークな利点は標識と精製オプションにあります。各AbFlex抗体にはSortase認識モチーフ(LPXTG)が含まれています。このSortase認識モチーフは蛍光物質酵素基質(HRPAPなど)ペプチドDNA薬物または他の標識を,直接的かつ再現性のある方法でこの抗体に共有結合により付加することができます。すべての抗体はニッケルベースの精製システムと一緒に使用することが可能な,6xHisタグおよびビオチンリガーゼ BirAを用いたビオチン結合のためのアビジンタグ配列も含んでいます。

AbFlex antibody with flexible labeling and purification options
 

AbFlex®リコンビナント抗体(rAb)は様々な標識と精製オプションを備えています

AbFlex抗体は,抗原認識および機能への干渉を避けるために,重鎖の定常領域の末端が特異的に標識されています。これは標識化プロセスが抗体の完全性を維持することを保証し,シグナルが非機能性抗体の結果として減少しないことにおいて重要です。対照的に、一般的に使用される化学的標識方法は、抗体にランダムに標識を加えます。このランダムなプロセスは,抗原結合部位をブロックし、抗体を無効化する可能性が高くなります。また化学的標識は,プロテインAとの相互作用を妨げる可能性のある抗体のFc領域に標識を付けることもあります。

AbFlex directed labeling versus chemical labeling

AbFlex®直接標識と化学標識との標識配置の違い 

リコンビナント抗体開発以前は,研究者はポリクローナルおよびモノクローナル抗体に依存していました。ポリクローナル抗体は,同じ抗原上の複数のエピトープを認識します。これは抗体親和性を増加させますが,しばしば交差反応性の可能性が高くなります。モノクローナル抗体は,一つのユニークなエピトープに結合するB細胞を産生する単一抗体です。しかしながら,モノクローナル抗体は,時間的経過で失われたり,進化したりする可能性があります。

モノクローナル抗体は,抗体ロット全体の可変性をもたらし,時間的経過で抗体の性能を変化させる遺伝子欠損,突然変異および細胞株ドリフトを起こす可能性がありますが,リコンビナント抗体はモノクローナル抗体とは異なり,どのロットも同じ免疫グロブリン重鎖および軽鎖のDNA配列を含むため,高い一貫性があります。これにより,抗体性能のバッチ間の再現性が得られ,異なる抗体ロットを再評価または比較する必要がなくなることで,アッセイ開発に貴重な時間と労力を節約できます。

最適な性能を保証するために,各AbFlex抗体は,特異性および感度について注意深くスクリーニングが行われています。弊社のビーズベースの結合アッセイにおいて確認されたように,非常に特異的な抗体を産生する重鎖および軽鎖配列のみが選択されます。 またAbFlex直接標識アプローチを利用する抗体も,様々なアプリケーションにわたって親モノクローナル抗体よりも高い感受性を示します。

AbFlexリコンビナント抗体の別の利点として,アニマルフリーで生産されることです。ポリクローナル抗体は、動物の免疫応答およびエピトープ特異性に依存しており,両方とも動物および免疫化によって非常に変化します。またポリクローナル抗体は供給の限界があるのため,長期間の抗体プロジェクトの実施や時間的経過または研究室間にわたって同様なデータを再現することは困難になります。 AbFlexリコンビナント抗体では,完全長IgGは哺乳動物系で発現させることで,一貫して安定な抗体供給を保証します。さらに抗体産生および維持に関連する倫理的または動物愛護上の懸念が回避されます。

AbFlex®リコンビナント抗体(rAb)の利点

  • 標識および精製オプション
  • ロット間での一貫した性能
  • 高い特異性および高感度抗体
  • 信頼性の高い長期供給
  • アニマルフリー生産

以下のリストは,アクティブ・モティフ社AbFlexリコンビナント抗体(rAb)です。
 
Name Isotype Reactivity Appl. Format Cat No. 価格 (税抜)  
AbFlex® 5-methylcytosine antibody (rAb) IgG2a Other (Not Species Specific) DB 100 µg 91187 ¥62,000 Buy Now
10 µg 91188 ¥17,000 Buy Now
AbFlex® Ago 1/2/3 antibody (rAb) IgG2a Human IP 100 µg 91217 ¥62,000 Buy Now
10 µg 91218 ¥17,000 Buy Now
AbFlex® AM-Tag antibody (rAb) IgG2a Other (Wide Range) ChIP, ELISA, WB 100 µg 91111 ¥62,000 Buy Now
10 µg 91112 ¥17,000 Buy Now
AbFlex® AM-Tag antibody (rAb) (biotin) IgG2a Other (Wide Range) ChIP, ELISA, WB 100 µg 91109 ¥64,000 Buy Now
10 µg 91110 ¥18,000 Buy Now
AbFlex® ATM phospho Ser1981 antibody (rAb) IgG2a Human, Mouse DB, WB 100 µg 91207 ¥62,000 Buy Now
10 µg 91208 ¥17,000 Buy Now
AbFlex® BMI-1 antibody (rAb) IgG2a Human WB 100 µg 91195 ¥62,000 Buy Now
10 µg 91196 ¥17,000 Buy Now
AbFlex® BrdU antibody (rAb) IgG2a Other (Wide Range) IF 100 µg 91269 ¥62,000 Buy Now
10 µg 91270 ¥17,000 Buy Now
AbFlex® Cas9 antibody (rAb) IgG2a Human ELISA, WB 100 µg 91123 ¥62,000 Buy Now
10 µg 91124 ¥17,000 Buy Now
AbFlex® SaCas9 antibody (rAb) IgG2a Other (Not Species Specific) WB 100 µg 91279 ¥62,000 Buy Now
10 µg 91280 ¥17,000 Buy Now
AbFlex® CHD3 antibody (rAb) IgG2a Drosophila IP, WB 100 µg 91273 ¥62,000 Buy Now
10 µg 91274 ¥17,000 Buy Now
AbFlex® Dicer antibody (rAb) IgG2a Mouse WB 100 µg 91241 ¥62,000 Buy Now
10 µg 91242 ¥17,000 Buy Now
AbFlex® EED antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA 100 µg 91135 ¥62,000 Buy Now
10 µg 91136 ¥17,000 Buy Now
AbFlex® EHMT2 (G9a) antibody (rAb) IgG2a Human WB 100 µg 91229 ¥62,000 Buy Now
10 µg 91230 ¥17,000 Buy Now
AbFlex® HA-Tag antibody (rAb) IgG2a Other (Not Species Specific) IP 100 µg 91271 ¥62,000 Buy Now
10 µg 91272 ¥17,000 Buy Now
AbFlex® HDAC1 antibody (rAb) IgG2a Human, Mouse ChIP-Seq, WB 100 µg 91215 ¥62,000 Buy Now
10 µg 91216 ¥17,000 Buy Now
AbFlex® HDAC2 antibody (rAb) IgG2a Human ChIP-Seq, WB 100 µg 91197 ¥62,000 Buy Now
10 µg 91198 ¥17,000 Buy Now
AbFlex® HDAC3 antibody (rAb) IgG2a Human WB 100 µg 91199 ¥62,000 Buy Now
10 µg 91200 ¥17,000 Buy Now
AbFlex® HIF-1 alpha antibody (rAb) IgG2a Bovine, Human, Mouse, Rat ELISA 100 µg 91139 ¥62,000 Buy Now
10 µg 91140 ¥17,000 Buy Now
AbFlex® His-Tag antibody (rAb) IgG2a Other (Not Species Specific) WB 100 µg 91183 ¥62,000 Buy Now
10 µg 91184 ¥17,000 Buy Now
AbFlex® Histone H3K4me0 antibody (rAb) IgG2a Human, Other (Wide Range) DB, WB 100 µg 91267 ¥62,000 Buy Now
10 µg 91268 ¥17,000 Buy Now
AbFlex® Histone H3K4me3 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, DB, WB 100 µg 91263 ¥62,000 Buy Now
10 µg 91264 ¥17,000 Buy Now
AbFlex® Histone H3K9me0 Antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91155 ¥62,000 Buy Now
10 µg 91156 ¥17,000 Buy Now
AbFlex® Histone H3K9ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91103 ¥62,000 Buy Now
10 µg 91104 ¥17,000 Buy Now
AbFlex® Histone H3K9ac antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91101 ¥64,000 Buy Now
10 µg 91102 ¥18,000 Buy Now
AbFlex® Histone H3K27ac antibody (rAb) IgG2a Human ChIP-Seq, ELISA, WB 100 µg 91193 ¥62,000 Buy Now
10 µg 91194 ¥17,000 Buy Now
AbFlex® Histone H3K36me3 antibody (rAb) IgG2a Other (Wide Range) ChIP, ChIP-Seq, WB 100 µg 91265 ¥62,000 Buy Now
10 µg 91266 ¥17,000 Buy Now
AbFlex® Histone H3K56ac antibody (rAb) IgG2a Human, Other (Wide Range) ChIP-Seq, ELISA 100 µg 91127 ¥62,000 Buy Now
10 µg 91128 ¥17,000 Buy Now
AbFlex® Histone H3.3 antibody (rAb) IgG2a Human ChIP-Seq, WB 100 µg 91191 ¥62,000 Buy Now
10 µg 91192 ¥17,000 Buy Now
AbFlex® Histone H4K20me3 antibody (rAb) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91107 ¥62,000 Buy Now
10 µg 91108 ¥17,000 Buy Now
AbFlex® Histone H4K20me3 antibody (rAb) (biotin) IgG2a Human, Other (Wide Range) ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91105 ¥64,000 Buy Now
10 µg 91106 ¥18,000 Buy Now
AbFlex® JMJD2A antibody (rAb) IgG2a Human ELISA, WB 100 µg 91143 ¥62,000 Buy Now
10 µg 91144 ¥17,000 Buy Now
AbFlex® KDM5A antibody (rAb) IgG2a Human ChIP-Seq, WB 100 µg 91211 ¥62,000 Buy Now
10 µg 91212 ¥17,000 Buy Now
AbFlex® Lamin A/C antibody (rAb) IgG2a Human IF, WB 100 µg 91189 ¥62,000 Buy Now
10 µg 91190 ¥17,000 Buy Now
AbFlex® MAZ antibody (rAb) IgG2a Human WB 100 µg 91205 ¥62,000 Buy Now
10 µg 91206 ¥17,000 Buy Now
AbFlex® MEIS 1/2/3 antibody (rAb)  IgG2a Human, Mouse WB 100 µg 91259 ¥62,000 Buy Now
10 µg 91260 ¥17,000 Buy Now
AbFlex® Mi-2 beta (CHD4) antibody (rAb) IgG2a Human WB 100 µg 91275 ¥62,000 Buy Now
10 µg 91276 ¥17,000 Buy Now
AbFlex® Mili / PiwiL2 antibody (rAb) IgG2a Mouse WB 100 µg 91253 ¥62,000 Buy Now
10 µg 91254 ¥17,000 Buy Now
AbFlex® MITF antibody (rAb) IgG2a Human ChIP-Seq 100 µg 91201 ¥62,000 Buy Now
10 µg 91202 ¥17,000 Buy Now
AbFlex® MPP8 antibody (rAb) IgG2a Human WB 100 µg 91277 ¥62,000 Buy Now
10 µg 91278 ¥17,000 Buy Now
AbFlex® Myc-Tag antibody (rAb) IgG2a Human WB 100 µg 91203 ¥62,000 Buy Now
10 µg 91204 ¥17,000 Buy Now
AbFlex® c-Myc antibody (rAb) IgG2a Human WB 100 µg 91213 ¥62,000 Buy Now
10 µg 91214 ¥17,000 Buy Now
AbFlex® Nanog antibody (rAb) IgG2a Mouse WB 100 µg 91251 ¥62,000 Buy Now
10 µg 91252 ¥17,000 Buy Now
AbFlex® Notch1 antibody (rAb) IgG2a Human WB 100 µg 91243 ¥62,000 Buy Now
10 µg 91244 ¥17,000 Buy Now
AbFlex® Nucleolin antibody (rAb) IgG2a Canine, Human WB 100 µg 91231 ¥62,000 Buy Now
10 µg 91232 ¥17,000 Buy Now
AbFlex® p53 antibody (rAb) IgG2a Human WB 100 µg 91247 ¥62,000 Buy Now
10 µg 91248 ¥17,000 Buy Now
AbFlex® PBX2 antibody (rAb) IgG2a Human, Mouse WB 100 µg 91227 ¥62,000 Buy Now
10 µg 91228 ¥17,000 Buy Now
AbFlex® Pim2 antibody (rAb) IgG2a Human WB 100 µg 91239 ¥62,000 Buy Now
10 µg 91240 ¥17,000 Buy Now
AbFlex® Rad21 antibody (rAb) IgG2a Human, Mouse IF, WB 100 µg 91245 ¥62,000 Buy Now
10 µg 91246 ¥17,000 Buy Now
AbFlex® RNA Pol II antibody (rAb) IgG2a Human ChIP-Seq, IF, WB 100 µg 91151 ¥62,000 Buy Now
10 µg 91152 ¥17,000 Buy Now
AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb) IgG2a Human ELISA, IF, WB 100 µg 91219 ¥62,000 Buy Now
10 µg 91220 ¥17,000 Buy Now
AbFlex® RNA pol II CTD phospho Ser2 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91115 ¥62,000 Buy Now
10 µg 91116 ¥17,000 Buy Now
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91119 ¥62,000 Buy Now
10 µg 91120 ¥17,000 Buy Now
AbFlex® RNA pol II CTD phospho Ser5 antibody (rAb) (biotin) IgG2a Human ChIP, ChIP-Seq, ELISA, ICC, IF, WB 100 µg 91117 ¥64,000 Buy Now
10 µg 91118 ¥18,000 Buy Now
AbFlex® SMARCA1 / SNF2L1 antibody (rAb) IgG2a Human WB 100 µg 91225 ¥62,000 Buy Now
10 µg 91226 ¥17,000 Buy Now
AbFlex® Snail antibody (rAb) IgG2a Human WB 100 µg 91255 ¥62,000 Buy Now
10 µg 91256 ¥17,000 Buy Now
AbFlex® TET1 antibody (rAb) IgG2a Human, Mouse WB 100 µg 91171 ¥62,000 Buy Now
10 µg 91172 ¥17,000 Buy Now
AbFlex® Ubiquitin antibody (rAb) IgG2a Other (Wide Range) WB 100 µg 91257 ¥62,000 Buy Now
10 µg 91258 ¥17,000 Buy Now

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex antibody with flexible labeling and purification options
AbFlex® recombinant antibody (rAb) with flexible labeling and purification options.

AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

AbFlex® directed labeling versus chemical labeling
Differences in label distribution between AbFlex® direct labeling and chemical labeling.

 

Prior to the development of recombinant antibodies, researchers relied on polyclonal and monoclonal antibodies. Polyclonal antibodies recognize multiple epitopes on the same antigen. This increases antibody affinity, but often has a higher potential for cross-reactivity. Monoclonal antibodies represent a single antibody producing B cell that binds to one unique epitope. However, monoclonal antibodies can be lost or evolve over time.

Unlike monoclonal antibodies, which can experience gene loss, mutations and cell-line drift that alter antibody performance over time and lead to variability across antibody lots, recombinant antibodies are highly consistent because they contain the same immunoglobulin heavy and light chain DNA sequences in every lot. This provides batch-to-batch reproducibility in antibody performance and eliminates the need to re-evaluate or compare different antibody lots, thereby, saving valuable time and effort on assay development.

To ensure optimal performance, each AbFlex® antibody is carefully screened for specificity and sensitivity. Only heavy and light chain sequences that produce highly specific antibodies, as verified in our bead-based binding assay, are selected. Antibodies that utilize the AbFlex® direct labeling approach also show greater sensitivity than their parent monoclonal antibodies across a variety of applications.

Another advantage of AbFlex® recombinant antibodies is the fact that they are produced outside of an animal system. Polyclonal antibodies rely on an animal’s immune response and epitope specificity, both of which are highly variable across animals and immunizations. Polyclonal antibodies are also hindered by having a finite supply, making it difficult to perform long-term antibody projects or reproduce data over time or across labs. With AbFlex® recombinant antibodies, the full-length IgG is expressed in a mammalian system to ensure a consistent, stable antibody supply. Additionally, any ethical or animal welfare concerns associated with antibody production and maintenance are avoided.

The following list contains Active Motif's AbFlex® recombinant antibodies (rAbs). For information about site-specific labeling of the AbFlex® antibodies, please visit our collection of Sortag-IT™ Labeling Kits.