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Bridging Antibody for Mouse IgG

免疫沈降におけるマウス一次抗体の親和性の上昇

マウス抗体の一部のアイソタイプは、標準的な免疫沈降およびクロマチン免疫沈降実験で用いられる,プロテインG共役アガロースビーズ/磁気ビーズと十分に結合しません。これは,プロテインGビーズとマウス一次抗体の両方と強い親和性で結合する,抗マウスIgG架橋抗体(anti-mouse IgG bridging antibody)を使用することによって改善されます。プロテインGビーズのためのマウス一次抗体の親和性の増加により、マウスIgG用架橋抗体の使用は,マウス一次抗体を使う全てのChIPおよび免疫沈降実験の結果を改善するのを助けます。

 
Name Format Cat No. 価格 (税抜)  
Bridging Antibody for Mouse IgG 500 µg 53017 ¥21,000 Buy Now

The Bridging Antibody for Mouse IgG helps improve immunoprecipitation (ChIP and IP) experiments that use primary mouse antibody by improving the affinity of the mouse primary antibody for the protein G-conjugated agarose/magnetic beads that are used in immunoprecipitation (Figure 1).

 
 
Figure 1: Improvement in ChIP using the Bridging Antibody for Mouse IgG.

ChIP was performed using chromatin from U-937 cells induced with TNF-α (10 ng/ml for one hour). PCR was performed with primers corresponding to the human IL-8 promoter.

Lanes 1-4: Beads pre-incubated with no bridging antibody.
Lanes 5-8: Beads pre-incubated with 5 µg bridging antibody.
Lanes 1, 2, 5 & 6: ChIP performed using NFκB p65 mouse monoclonal antibody, 2 µg per IP.
Lanes 3, 4, 7 & 8: ChIP performed using negative control mouse IgG.

Contents & Storage

Five hundred µg of affinity-purified anti-mouse IgG in PBS, 30% glycerol and 0.035% sodium azide. Store at -20°C. Guaranteed stable for 6 months when stored properly.