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Fully Methylated Jurkat DNA

Fully Methylated Jurkat DNA はDNAメチル化解析の際にコントロールとしてご使用頂けます。 バイサルファイトシーケンス解析, メチル化特異的PCR (MSP), メチル化DNAの免疫沈降 (MeDIP) を行う際, またはMethylCollector™ や MethylDetector™ Kit 使用時に, Fully Methylated Jurkat DNA はCpG ジヌクレオチドメチル化解析の簡便なポジティブコントロールとなります。

Fully Methylated Jurkat DNA には BRCA1 プライマーセットが含まれます。通常, Jurkat DNA はBRCA1 遺伝子座ではメチル化されていないため, このプライマーセットは, Fully Methylated Jurkat DNAと使用することで最適なコントロールとしてご使用頂けます。

 
Name Format Cat No. 価格 (税抜)  
Fully Methylated Jurkat DNA 10 µg 55003 ¥47,000 Buy Now
Jurkat genomic DNA 10 µg 55007 ¥13,000 Buy Now
 
 
Figure 1: Fully Methylated Jurkat DNA used as a control for MethylCollector™.

The MethylCollector Kit was used to assay 100 ng genomic DNA (lanes 2 & 4) and Fully Methylated Jurkat DNA (lanes 3 & 5) samples with (lanes 4 & 5) or without (lanes 2 & 3) inclusion of 1 µg of the kit’s His-tagged MBD2b protein. These samples, the input genomic DNA (lane 6) and the input Fully Methylated Jurkat DNA (lane 7) were then PCR amplified with the BRCA1 primers. Lane 8 is a water-only PCR control. A positive result is observed only with Fully Methylated Jurkat DNA captured by MethylCollector’s His-tagged MBD2b (lane 5), demonstrating that MethylCollector specifically isolates methylated DNA and that Fully Methylated Jurkat DNA is a useful control in methylation-specific experiments.

Contents & Storage

10 µg of Fully Methylated Jurkat DNA (200 µl at 50 ng/µl), and BRCA1 PCR Primer Mix. Store at -20°C. All reagents guaranteed for 6 months when stored properly.

Jurkat genomic DNA contains 10 µg (50 ng/µl) undigested genomic DNA.