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for Recombinant c-Myc proteinContents
5 µg of Recombinant c-Myc protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM (NH4)2SO4, 2 mM DTT and 0.5 mM EDTA). Protein is supplied at 0.5 µg/µl in Dilution Buffer AM1.
for Recombinant c-Myc proteinApplication Notes
Recombinant c-Myc protein is suitable for DNA and protein-protein interaction assays. 100 ng are sufficient for DNA-protein and protein-protein interaction studies. The standard curve for TransAM® c-Myc was generated using the range of 5-0.625 ng of protein. The molecular weight of the protein is ~64 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
for Recombinant c-Myc proteinProtein Details
Recombinant c-Myc was expressed in a bacterial system (accession number NM 002467) with an amino terminal polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 75% homogeneous and contains no detectable protease, DNase and RNase activity.
TransAM® standard curve generated using Recombinant c-Myc protein.
The standard curve for TransAM® was generated using a range of 5-0.625 ng of protein and run on the TransAM® c-Myc ELISA Kit.
for Recombinant c-Myc proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
