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Histone H2BK5ac antibody (pAb)
| RRID: AB_2615079 |
| Aliases: Histone H2B acetyl Lysine 5 |
| 商品コード: 39123 | Format: 200 µl | ¥69,000 | Buy |
| 商品コード: 39124 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-Seq
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H2B acetyl Lys5 antibody was raised against a peptide including acetyl-lysine 5 of human histone H2B.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H2B acetyl Lys5 pAb tested by ChIP-Seq.
ChIP was performed using chromatin from the H1 human embryonic stem cell line. ChIP DNA was sequenced on the Illumina GA II and sequence tags were mapped to identify H2BK5Ac binding. The image shows H2BK5Ac binding across a 1 MB region on chromosome 1.
Histone H2B acetyl Lys5 pAb tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2B acetyl Lys5 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Detection of H2BK5ac by immunofluorescence.
U2OS cells were stained with H2BK5ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2BK5ac antibody staining. Right panel: merge.
Histone H2B acetyl Lys5 pAb tested by Western blot.
HeLa acid extract (5 µg per lane) was probed with Histone H2B acetyl Lys5 polyclonal antibody (1:2,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.
Lane 3: Human recombinant H2B (200 ng).
Histone H2B acetyl Lys5 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys5 pAb for acetyl-Lys5 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000.
Lane 1: Peptide acetylated at lysine 5 of H2B.
Lane 2: Unmodified lysine 5 peptide.
Lane 3: Peptide acetylated at lysine 16 of H2B.
Lane 4: Unmodified lysine 16 peptide.
Lane 5: Peptide acetylated at lysine 120 of H2B.
Lane 6: Unmodified lysine 120 peptide.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2BK5ac antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
