Histone H3K14me2 antibody (pAb)

RRID: AB_2793225
商品コード: 39349 Format: 200 µl ¥66,800 Buy
商品コード: 39350 Format: 10 µl ¥18,800 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 dimethyl Lys14 antibody was raised against a peptide containing dimethyl-lysine 14 of human histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3K14me2 antibody (pAb) tested by Western blot.

Histone H3 dimethyl Lys14 pAb tested by Western blot.
Detection of dimethylated Histone H3 by Western blot. The analysis was performed using 20 µg HeLa acid extract (10 µg, lane 1) and Histone H3 dimethyl Lys14 pAb at a 1:500 dilution.

Histone H3K14me2 antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Lys14 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys14 antibody for histone H3 dimethylated at Lys14. Methylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated in the left lane next to each row.
Top panel: Lane 1: Unmodified lysine 4. Lane 2: Monomethyl lysine 4. Lane 3: Dimethyl lysine 4. Lane 4: Trimethyl lysine 4. Lane 5: Monomethyl lysine 4 #2. Lane 6: Unmodified corresponding to amino acids 6-22 of human histone H3. Lane 7: Monomethyl lysine 9. Lane 8: Dimethyl lysine 9. Lane 9: Trimethyl lysine 9. Bottom panel: Lane 1: Unmodified lysine 27. Lane 2: Monomethyl lysine 27. Lane 3: Dimethyl lysine 27. Lane 4: Trimethyl lysine 27. Lane 5: Monomethyl lysine 14 #2. Lane 6: Dimethyl lysine 14. No detection of peptides (mono-, di-, or trimethylated) corresponding to lysine 56 of histone H3 was observed (data not shown).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting