Histone H3K122me1 antibody (pAb)

RRID: AB_2793230
商品コード: 39367 Format: 200 µl ¥66,800 Buy
商品コード: 39368 Format: 10 µl ¥18,800 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Immunogen

This Histone H3 monomethyl Lys122 antibody was raised against a peptide containing monomethyl-lysine 122 of human histone H3.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3K122me1 antibody (pAb) tested by Western blot.

Histone H3 monomethyl Lys122 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with Histone H3 monomethyl Lys122 pAb (1:500 dilution).

Histone H3K122me1 antibody (pAb) tested by dot blot analysis.

Histone H3 monomethyl Lys122 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of 39367 for monomethyl Lys122 histone H3. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with 39367 at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: unmodified Lys122 H3 peptide.
    Lane 2: monomethyl-Lys122 H3 peptide.
    Lane 3: dimethyl-Lys122 H3 peptide.
    Lane 4: trimethyl-Lys122 H3 peptide.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting